Nanoparticle delivery of Cas9 ribonucleoprotein and donor DNA in vivo induces homology-directed DNA repair
نویسندگان
چکیده
منابع مشابه
Targeted gene knock-in by homology-directed genome editing using Cas9 ribonucleoprotein and AAV donor delivery
Realizing the full potential of genome editing requires the development of efficient and broadly applicable methods for delivering programmable nucleases and donor templates for homology-directed repair (HDR). The RNA-guided Cas9 endonuclease can be introduced into cells as a purified protein in complex with a single guide RNA (sgRNA). Such ribonucleoproteins (RNPs) can facilitate the high-fide...
متن کاملthe convenience of single homology arm donor dna and crispr/cas9-nickase for targeted insertion of long dna fragment
objective: crispr/cas9 technology provides a powerful tool for targeted modification of genomes. in this system, a donor dna harboring two flanking homology arms is mostly used for targeted insertion of long exogenous dna. here, we introduced an alternative design for the donor dna by incorporation of a single short homology arm into a circular plasmid. materials and methods: in this experiment...
متن کاملNoncanonical views of homology-directed DNA repair.
DNA repair is essential to maintain genomic integrity and initiate genetic diversity. While gene conversion and classical nonhomologous end-joining are the most physiologically predominant forms of DNA repair mechanisms, emerging lines of evidence suggest the usage of several noncanonical homology-directed repair (HDR) pathways in both prokaryotes and eukaryotes in different contexts. Here we r...
متن کاملDNA Damage, Homology-Directed Repair, and DNA Methylation
To explore the link between DNA damage and gene silencing, we induced a DNA double-strand break in the genome of Hela or mouse embryonic stem (ES) cells using I-SceI restriction endonuclease. The I-SceI site lies within one copy of two inactivated tandem repeated green fluorescent protein (GFP) genes (DR-GFP). A total of 2%-4% of the cells generated a functional GFP by homology-directed repair ...
متن کاملCorrection: DNA Damage, Homology-Directed Repair, and DNA Methylation
[This corrects the article DOI: 10.1371/journal.pgen.0030110.].
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ژورنال
عنوان ژورنال: Nature Biomedical Engineering
سال: 2017
ISSN: 2157-846X
DOI: 10.1038/s41551-017-0137-2